Avidin binds biotin with a Kd of ~10?15 mol/L. A protein of interest can be covalently linked to biotin and subsequently isolated by incubating with beads coated with avidin
If the concentration of biotinylated protein is 10-8 M at the beginning of the assay and the beads provided an excess of avidin-binding sites, then one could expect the beads to bind:
A. a small fraction of the protein in the sample.
B. approximately half the protein in the sample.
C. almost all of the protein in the sample.
D. 10–15 mol of protein per 1 mL of sample.
E. This cannot be predicted.
C
You might also like to view...
A defining characteristic of eukaryotic organisms is that they
A. have their transcription occurring in the cytoplasm and translation in the nucleus. B. have their transcription occurring in the nucleus and translation in the cytoplasm. C. have only operons to assist in gene expression. D. carry out protein synthesis only in the presence of the cAMP molecule. E. use the leucine zipper primarily for the production of the amino acid tryptophan.
A patient with a viral infection would typically demonstrate a decline in the number of agranulocytic leukocytes in circulating blood with an accompanying increase in the number of granulocytic white blood cells, such as basophils and eosinophils.
Answer the following statement true (T) or false (F)
The questions that can be answered by science are:
A) limited by religious doctrine. B) without limit. C) limited by what is found in the living world. D) limited by what can be investigated using the scientific method. E) limited only by imagination.
Two genes are on the same chromosome, yet crossing over occurs between them every time meiosis
occurs. Are these genes linked or unlinked and why?
What will be an ideal response?