Histone proteins are the principal agents in chromatin packaging. The nucleosome core particle is a heterooctameric protein complex that contains two molecules each of the four histones H2A, H2B, H3, and H4

Describe the process of managing existing nucleosome core particles during replication and the process of adding these and new nucleosome core particles to DNA after the replication fork passes.


The assembly of nucleosome core particles in connection with replication is driven by the partial denaturing of old (previously used) core particles into either dimers (H2A-H2B) or tetramers (H3-H4). These old core particle components are randomly joined with other dimers and tetramers after replication to form complete nucleosome core particles. New histone proteins are also available during replication that also form dimers and tetramers. A mixture of old and newly synthesized core particles is the pool from which post-replication core particles are assembled.

Biology & Microbiology

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