Which of the following is an acceptable sample for testing on a photo-optical clot-detecting instrument?
a. Lipemic plasma
b. Hemolyzed plasma
c. Serum
d. Plasma collected using a 9:1 ratio of whole blood to 3.2% sodium citrate
ANS: D
Plasma should be collected at a 9:1 ratio of blood to anticoagulant (3.2% sodium citrate). Lipemia and hemolysis are possible interfering substances when using an instrument that uses the photo-optical principle for end-point detection. Serum cannot be used for coagulation testing because the sample has already clotted, with resultant loss of several coagulation proteins.
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