Place the basic steps involved in a molecular hybridization assay in sequence from first to last in the order in which they would be performed
a. Heating sample to obtain single-stranded target nucleic acid, hybridization of target to probe, detection of hybridization, labe-ling of probe
b. Labeling of probe, heating sample to ob-tain single-stranded target nucleic acid, hybridization of target to probe, detection of hybridization
c. Hybridization of target to probe, detection of hybridization, labeling of probe, heating sample to obtain single-stranded target nucleic acid
d. Hybridization of target to probe, labeling of probe, heating sample to obtain sin-gle-stranded target nucleic acid, detection of hybridization
B
The basic steps in a hybridization assay include:
1 . Production and labeling of single-strand nucleic acid probe
2 . Preparation of single-strand target nucleic acid
3 . Mixture and hybridization of target and probe nucleic acid
4 . Detection of hybridization
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