Which of the following is not a component of a yeast three-hybrid analysis?
A. a fusion protein of LexA, which binds the reporter construct, and MS2, which specifically binds an RNA tag that has been added to every RNA produced by the plasmid library
B. a plasmid library that contains random sequences from the genome tagged with a unique hairpin sequence that MS2 will bind
C. a reporter construct that contains a LexA operator (binding site) and a reporter gene; activation of the reporter gene is either required for growth of the bacteria or catalyzes a reaction that forms a colored product
D. a fusion protein containing the protein of interest fused to the activation domain that will activate transcription of the reporter gene when bound
E. a fusion protein of the protein of interest and two tags?the calmodulin binding site and protein A
E
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We can conclude that natural selection must have acted in favor of large brains in humans because:
A. we have large brains in proportion to our body size compared to other mammals. B. large brains have allowed us to dominate the planet. C. large brains are metabolically expensive to produce and maintain. D. we would not have large brains if natural selection did not favor them. E. None of the answer options are correct.
If you were following the inheritance patterns of two different sets of multiple alleles located on
different chromosomes, how many different possible gametes could be produced if locus 1 had five possible alleles and locus 2 had six alleles? What will be an ideal response?
For a particular chemical reaction, the enthalpy of the reactants is -400 kJ. The enthalpy of the products is -390 kJ. The entropy of the reactants is 0.2 kJ/K. The entropy of the products is 0.3 kJ/K. The temperature of the reaction is 25 degrees Celsius. What can you conclude about this reaction?
A. it is a redox reaction B. It is being catalyzed by an enzyme C. It is endergonic D. It is exergonic
The use of chemical agents directly on exposed body surfaces to destroy or inhibit vegetative pathogens is
A. sanitization. B. degermation. C. sterilization. D. disinfection. E. antisepsis.