The wet mount and Gram stain of vaginal secretions from a patient who has bacterial vaginosis caused by Gardnerella vaginalis will reveal large:
a. squamous epithelial cells with numerous attached pleomorphic gram-variable or gram-negative coccobacilli and rods.
b. numbers of large gram-positive rods.
c. numbers of polymorphonuclear leuko-cytes.
d. pleomorphic gram-negative rods with many leukocytes.
A
Gardnerella are small pleomorphic gram-variable or gram-negative coccobacilli and have short rods. The wet mount and Gram stain of vaginal secretions are key tests for diagnosing bacterial vaginosis caused by G. vaginalis. A wet mount prepared in saline solution reveals the characte-ristic "clue cells," which are large, squamous epithelial cells with numerous attached small rods. A Gram-stained smear of the discharge shows the attached organisms to be gram-variable coc-cobacilli. Clue cells typically are present, and large numbers of gram-positive rods (e.g., lactoba-cilli) representing normal vaginal flora are absent or few in number.
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