How were the sequences that compose the replication origins in yeast discovered?

What will be an ideal response?


Yeast cells were grown in heavy isotope media to produce denser DNA and then shifted to media with normal, light isotopes. After a few minutes, DNA from these cells was extracted and cut into small pieces. The lighter DNA fragments (which should contain the origins of replication) were collected, fluorescently labeled, and used to hybridize a microarray of yeast sequences. Determination of the sequences to which this light, fluorescently labeled DNA hybridized led to the identification of a number of yeast replication origins.

Biology & Microbiology

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Biology & Microbiology

A plant's ability to absorb carbon dioxide is reduced when:

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Biology & Microbiology

What is the name of the birth defect in which the neural tube fails to close completely?

a. neurulation b. fossa ovalis c. organogenesis d. spina bifida e. capacitation

Biology & Microbiology

A result that is very unlikely to have occurred by chance alone is said to be ________ .

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Biology & Microbiology