By what mechanism can CRISPR-Cas technology introduce a point mutation into a gene?
A. The double-strand break made by Cas9 is repaired by end-joining.
B. The activity of Cas9 changes the DNA sequence of its target.
C. The single guide RNA is incorporated into the eukaryotic genome by Cas9.
D. A double crossover replaces DNA cut by Cas9 with a donor DNA.
Answer: D
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