Provide the major steps in sequence to perform acid-fast staining (omit rinses)

What will be an ideal response?


Ans:
1. prepare heat fixed smear
2. Place absorbent on bacteria
3. Place a lot of carbofushin on top of smear and heat the slide, by placing it on top of a beaker in water
4. remove the absorbent paper
5. decolorize w/ acid alcohol
6. counterstain with methylene blue

Biology & Microbiology

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Answer for this statement?

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