Bacteria are grown in 15N (heavy) medium and then transferred to 14N (light) medium and are allowed to replicate for one generation. The DNA is subsequently isolated and centrifuged in a CsCl2 gradient to yield what type of gradient band(s)?

A. one heavy and one half-heavy band
B. one heavy and one light band
C. one light band
D. one half-heavy (intermediate to heavy and light) band
E. one heavy band


Answer: D

Biology & Microbiology

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