Based upon these laboratory results, what microbe do you predict is causing Caitlyn's infection? Explain. What two chemical tests would you perform next to verify your answer ?

What will be an ideal response.


Answer: Caitlyn’s infection is mainly caused by Staphylococcus bacteria because the Gram-stain was positive and Staphylococcus bacteria is a Gram-positive bacteria that is found on the skin and causes skin infections. To verify, the sample could be isolated using a Mannitol Salt Agar (MSA), which Gram-positive bacteria like Staphylococcuscan grow very well in. Also the bacteria could be identified via a catalase test as it is useful to distinguish Staphylococci from enterococci and Streptococci. To confirm the species of Staphylococcus, a coagulase test, can be used because Staphylococcus epidermidis does not produce free coagulase while Staphylococcus aureus does not. Colonies from the TSA plate were suspended in sterile saline and introduced into the Vitek II analyzer. It confirmed Staphylococcus epidermidis was the pathogen involved and indicated Ke?ex sensitivity.

Biology & Microbiology

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