Which of the following statements is true about the decolorizing step for the acid-fast stain?

A) Because acid-fast bacteria have a waxy cell wall that resists decolorization by the acetone-alcohol rinse, they appear a deep blue at the end of the procedure.
B) Over-decolorizing can lead to false-positive results.
C) Acetone-alcohol solution is the decolorizing agent.
D) The decolorizing step is the differentiating step.
E) Non-acid-fast bacteria appear bright pink-red at the end of the procedure.


Answer: D

Biology & Microbiology

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