Using Northern and Southern blot techniques, you study a gene, CAE1, that seems to be associated with lung cancer. Your probe in both blots is a 32P-labeled single strand DNA that can base pair with the CAE1 RNA and with the CAE1 gene

Question

You isolate DNA and RNA from brain, heart, lung, and cancerous lung tissue. The DNAwas cut into DNA fragments with a restriction endonuclease before electrophoresis. The results of the blots are shown here.

Which one of the following is an observation or conclusion consistent with the data?

A. There are fewer CAE1 genes in brain tissue compared to heart tissue.
B. The DNA sequence of the CAE1 gene in lung cancer cells is different than in other tissues.
C. The amount of CAE1 RNA in lung cancer cells is higher than in other tissues.
D. The amounts of all RNAs in heart cells and normal lung cells are the same.
E. Deleting the CAE1 gene in all tissue types would be a possible cancer cure with little or no side effects.

Answer 1

C