Which cloning vector should be used to express a 500-kb DNA fragment in Saccharomyces cerevisiae?

A. ?1059
B. BAC
C. YAC
D. pUC19


Answer: C

Biology & Microbiology

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An enzyme synthesized in the laboratory is found to have little activity when compared to the enzyme extracted from cell culture. Both enzymes were examined and have identical amino acid composition

What is the best explanation for the lack of activity of the synthesized enzyme? A) The van der Waals radius was altered during laboratory synthesis. B) The synthetic enzyme was not made of amino acids. C) The ATP required for self-assembly was present in the cell extract but not in the laboratory synthesis. D) Denaturation of the synthesized enzyme was not complete. E) The synthesized enzyme was not folded correctly because molecular chaperones were not present.

Biology & Microbiology

Which of the following is in the coding region of a gene?

A. Trailer B. Leader C. Promoter D. None of the choices are correct.

Biology & Microbiology

Researchers have proposed that the herpes virus responsible for the childhood illness roseola may cause a type of T cell leukemia. Ninety percent of the population has been infected by the virus by two years of age, but the T cell leukemia is relatively

rare. The virus can be cultured in the laboratory in tissue culture, but not in mature organisms. Discuss the obstacles to applying Koch's postulates to the etiology of this disease. What will be an ideal response?

Biology & Microbiology

Teenagers are at risk of deficiency in which of the following nutrients?

Select one: a. Fats and protein b. Calcium and folate c. Calcium and iron d. Iron and sodium

Biology & Microbiology