Your research project involves the characterization of a particular signal transduction pathway. Specifically, you have been studying a receptor tyrosine kinase and protein X, which initiates downstream events. You are certain that protein X is part of the signal transduction pathway, but have been unable to identify a direct protein-protein interaction between the receptor tyrosine kinase and the protein X. What should you do next in your attempt to identify a link between the receptor tyrosine kinase and protein X?
A. try to identify an adapter protein that can interact with both the receptor and protein X
B. try to identify proteins that act downstream of protein X
C. confirm the interaction between the receptor tyrosine kinase and its ligand
D. try to identify a coactivator protein
Answer: A
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