Nucleic acid blotting is commonly used in molecular biology. Two types, Southern blots and Northern blots, involve gel electrophoresis and a filter, which holds the nucleic acid
Briefly describe the procedure of "blotting" in this context and differentiate between Southern and Northern blots.
In a Southern blot, the DNA to be "probed" is cut with a restriction enzyme(s); then the fragments are separated by gel electrophoresis. Alkali treatment of the gel denatures the DNA, which is then "blotted" onto the filter (nylon or nitrocellulose). A labeled probe (RNA or DNA) is then hybridized to complementary fragments on the filter. In a Northern blot, RNA is separated in the gel and "probed" with the labeled DNA.
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Mutations that result in the death of an organism when expressed are called ________ mutations.
A. recessive B. dominant C. conditional D. lethal
When you look at an image, it is inverted on the back of the retina and yet appears right-side-up when perceived in the brain. What part of the body is responsible for inverting the image?
Select one: a. the retina b. the rods and cones c. the optic nerve d. the brain e. the lens
Transgenic crop plants have been created that do all of the following except
a. grow two kinds of crops, like the pomato which produces both tomatoes and potatoes. b. are resistant to insect damage. c. are resistant to herbicides. d. produce human hormones or antibodies. e. transgenic plants have been produced that can do all of the things listed.
Which marine zone would have the lowest rates of primary productivity (photosynthesis)?
A) pelagic B) abyssal C) neritic D) continental shelf E) intertidal