Nucleic acid blotting is commonly used in molecular biology. Two types, Southern blots and Northern blots, involve gel electrophoresis and a filter, which holds the nucleic acid
Briefly describe the procedure of "blotting" in this context and differentiate between Southern and Northern blots.
In a Southern blot, the DNA to be "probed" is cut with a restriction enzyme(s); then the fragments are separated by gel electrophoresis. Alkali treatment of the gel denatures the DNA, which is then "blotted" onto the filter (nylon or nitrocellulose). A labeled probe (RNA or DNA) is then hybridized to complementary fragments on the filter. In a Northern blot, RNA is separated in the gel and "probed" with the labeled DNA.
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Mutations that result in the death of an organism when expressed are called ________ mutations.
A. recessive B. dominant C. conditional D. lethal
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Select one: a. the retina b. the rods and cones c. the optic nerve d. the brain e. the lens
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