The steps involved in a single PCR cycle, in the correct order, are

A. heat to denature DNA strands and for primer to anneal, cool to allow DNA polymerase to copy DNA.
B. heat to denature DNA strands, cool to allow primer to anneal, heat slightly to allow DNA polymerase to copy DNA.
C. cool to denature DNA strands, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
D. cool to allow DNA strands to anneal, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
E. cool to allow primer to anneal, heat to denature DNA strands, cool to allow DNA polymerase to copy DNA.


Answer: B

Biology & Microbiology

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