You have identified a molecule, molecule X, that can bind to the ATP binding site of a specific protein kinase, and prevent ATP binding. You are conducting a kinase assay using this protein kinase and a known substrate of this kinase, and have set up the reaction using the proper conditions and cofactors to allow for optimal protein kinase activity. If you add molecule X to the reaction, what do you predict will be the outcome?
A. More phosphorylation of the substrate will be seen in the presence of molecule X.
B. The substrate will be phosphorylated normally in the presence of molecule X.
C. Less phosphorylation of the substrate will be seen in the presence of molecule X.
Answer: C
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