A mouse genomic library is constructed so that the number of clones equals one genomic equivalent. Is it likely that this library contains the entire mouse genome?
The mouse genome consists of 2,700 Mb. A genomic library is constructed using BAC vectors and overlapping fragments of the mouse genome. The average size of the mouse DNA inserts is 200 kb.
A) No; by chance some parts of the genome will be represented more than once and others not at all.
B) Yes; that is a sufficient number clones for every part of the genome to be represented one time.
C) No; the restriction enzyme used to fragment the genome will delete some DNA on the ends of clones.
A) No; by chance some parts of the genome will be represented more than once and others not at all.
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What will be an ideal response?
ACTH
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