Pick the correct statement as it relates to hemoglobin and acid-base buffering
a. Deoxygenated hemoglobin acts as an acid at the tissue level.
b. Deoxygenated hemoglobin is a fairly strong base.
c. Hemoglobin contributes more H+ in the face of increased histidine.
d. In an alkaline environment, hemoglobin becomes an ineffective base.
ANS: B
The ability of proteins to accept hydrogen ions limits H+ activity in solution, which is called buffering. The ability of hemoglobin to accept (i.e., buffer) H+ ions depends on its oxygenation state. Deoxygenated (reduced) hemoglobin is a stronger base (i.e., a better H+ acceptor) than ox-ygenated hemoglobin.
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