You have evidence that a gene, VTE4, of known sequence and well-understood transcriptional regulation, is available from another lab
The product of this gene enables seeds to produce a significantly increased amount of vitamin E from a substrate already produced in Brassica napus, the plant that gives us canola oil. To produce canola oil with increased vitamin E, how would you proceed? A) Select Brassica plants with the highest titer of the vitamin, isolate the genes responsible, and cause these genes to duplicate.
B) Use a yeast system to engineer yeasts that produce the vitamin, and get manufacturers to add this to the canola oil during bottling.
C) Introduce the gene and necessary cis elements into Brassica, along with a reporter gene, then measure the vitamin E produced.
D) Clone VTE4 in A. tumefaciens and use these bacteria to produce transgenic Brassica.
E) Introduce VTE4 into tomato plants or another food that humans eat more than Brassica.
C
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