Cultures for isolation of Campylobacter jejuni and Campylobacter coli should be incubated in:

Question

a. a capnophilic atmosphere at 42°C and examined at 24 and 48 hours.
b. a microaerophilic atmosphere at 35°C and examined at 24 and 48 hours.
c. regular room air at 35°C and examined at 48 and 72 hours.
d. a microaerophilic atmosphere at 42°C and examined at 24 and 48 hours.

Answer 1

D
Cultures for isolation of C. jejuni and C. coli should be inoculated to a selective agar containing antimicrobial agents that suppress the growth of normal flora but not of Campylobacter spp. The introduction of a blood-free, charcoal-containing medium that has more selective antibiotic components has resulted in better recovery of most enteropathogenic Campylobacter spp., com-pared with earlier media. Commercially produced agar plates for isolation of Campylobacters are available from several manufacturers. These plates are incubated in a microaerophilic atmosphere at 42°C and examined at 24 and 48 hours for suspicious colonies.