Some restriction enzymes cleave DNA in such a manner as to produce blunt ends. Ligation of blunt end fragments is most often enhanced by the use of the enzyme terminal deoxynucleotidyl transferase

Speculate on the function of deoxynucleotidyl transferase in terms of using blunt end fragments in cloning.


Terminal deoxynucleotidyl transferase extends single-stranded ends by the addition of nucleotide tails. If complementary tails are added, the fragments can hybridize and the recombinant molecules can be ligated.

Biology & Microbiology

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Sometimes, one gene pair will interfere with the expression of a second gene pair in an interaction called  

A.  incomplete dominance. B.  codominance. C.  blending inheritance. D.  pleiotropy. E.  epistasis.

Biology & Microbiology

Nucleotides have a phosphate group attached at the ___ carbon atom of the sugar.  

A.  2' B.  3' C.  5' D.  1'

Biology & Microbiology

True or false: External fertilization is generally associated with large numbers of offspring and low levels of parental care

A. true B. false

Biology & Microbiology

What is an ecotone?

a. a parasite that is external to the body b. a parasite that is internal to the body c. an example of an individualistic hypothesis d. a zone between two communities e. the combination of species in an environment that is constant

Biology & Microbiology