You isolate a new bacterium from soil with a revolutionary new culture technique. You are interested in sequencing the genome of this organism using the whole-genome, or shotgun, sequencing approach

Of the following choices, what would be the best way to prepare the genomic DNA for sequencing?
A) digestion with HpaI (CCGG)
B) digestion with BamHI (GGATCC)
C) digestion with EcoRI (GAATTC)
D) digestion with NotI (GCGGCCGC)
E) digestion with EcoRI, BamHI, and HpaI


Answer: E

Biology & Microbiology

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Which of the following is the best reason that the origin of species according to creation science should not be included in the curriculum of biology courses? 

A. It is not a scientifically testable theory. B. It has proven to be false. C. It has been superseded by more modern theories. D. It is contrary to the beliefs of most scientists. E. It constitutes the teaching of religion.

Biology & Microbiology

After completion of a genome sequencing project, the DNA sequences are analyzed to locate and identify genes in a process called _____

a. automation b. comparative genomics c. physical mapping d. compilation e. annotation

Biology & Microbiology

All of the following allow for fast gas exchange EXCEPT

a. more dense medium. b. colder temperatures. c. a steep gradient. d. a large surface area.

Biology & Microbiology

Adaptations of thermophiles >55°C

What will be an ideal response?

Biology & Microbiology