Which of the following is not true about Kirby-Bauer disk diffusion method?
A. The diameters of the zones of inhibition are measured to the nearest millimeter.
B. The plate is placed at 35°C for 16 to 18 hours of incubation.
C. After inoculation of bacteria, the agar surface is dried for about 5 minutes and the appropriate antibiotic test disks are placed on it.
D. Bacteria used to swab plate should be at least a week old.
Answer: D
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