Actin filaments have polarity. This means that the two ends can be identified due to structural differences. The plus end is the end to which subunits are added more rapidly, or the end of polymerization
Which of the following would enable you to identify the plus end of actin filaments?
A) Add radiolabeled actin subunits to a mixture of actin filaments in which conditions are favorable for polymerization.
B) Add radiolabeled actin subunits to a mixture of actin filaments in which conditions favor depolymerization.
C) Determine the ionic charge of the ends of the actin filaments.
Answer: A
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