A student uses restriction enzymes to cut a DNA molecule into fragments. The digested DNA is loaded into the wells of an agarose gel and the gel is subjected to an electric current. Upon completion of the run, the gel is stained. The procedures described can be used to do all of the following EXCEPT
(A) isolate and purify certain DNA fragments
(B) synthesize novel DNA molecules
(C) study the activity of restriction enzymes
(D) calculate the size of DNA fragments
(E) identify the source of DNA material
Answer: (B) synthesize novel DNA molecules
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Why is it necessary to include the ?-galactosidase gene in a BAC?
A. ?-galactosidase is a negative selectable marker. B. ?-galactosidase is a positive selectable marker. C. ?-galactosidase confers resistance to several antibiotics. D. Presence of ?-galactosidase indicates cloned DNA segment is present. E. Absence of ?-galactosidase indicates cloned DNA segment is present.
When pyruvic acid is converted to acetyl CoA _____.
a. one turn of the Citric Acid cycle is completed b. CO2 and NADH are formed c. CO2 and ATP are formed d. NAD is regenerated e. CO2 and coenzyme A are formed
Which of the following is the only true statement concerning the structure at "C?"
a. filters water from the blood b. stores urine c. produces adrenalin d. concentrates urine e. reclaims glucose from urine
In primary endosymbiosis, an ancient eukaryotic cell engulfed a(n) ____, which survived and evolved into a
chloroplast. a. algae b. virus c. ciliate d. euglenoid e. cyanobacterium