A clinic nurse is preparing to sterilize instruments used in clinic procedures. The nurse will place the instruments in glutaraldehyde [Cidex-7]. Which actions are necessary when using this prod-uct? (Select all that apply.)
a. Acidifying the solution to improve its an-tiseptic properties
b. Cleansing the hands with an alcohol-based hand rub
c. Ensuring adequate ventilation in the room where the solution is used
d. Removing blood from the instruments before soaking them
e. Soaking the instruments for 10 hours or longer
C, D, E
To completely eliminate bacterial spores, all blood first should be removed from the instruments and equipment, which then should be immersed in glutaraldehyde for at least 10 hours. Because fumes from the solution can irritate the respiratory tract, adequate ventilation is essential. The solution works best at an alkaline pH. Cleansing the hands is always a good idea but is not ne-cessary to ensure proper sterilization of the instruments.
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