Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L-cysteine, sodium chloride, sodium thioglycolate, and agar. It is considered a complex medium because
A. depending on their oxygen utilization pattern, organisms will grow differently on it.
B. thioglycolate has a carbon-sulfur bond that many microbes cannot break enzymatically.
C. it has multiple ingredients for a broad range of nutrients.
D. both yeast extract and pancreatic digest of casein are not chemically defined.
Answer: D
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