The genes needed for ?-carotene synthesis are not normally expressed in endosperm. In the creation of golden rice, how did researchers ensure that these genes were expressed in rice endosperm?
A) The genes were injected only in the endosperm of rice grains.
B) Agrobacterium cells were added only to rice grains, not to any other part of the plant.
C) The protein-coding sequence of each gene was linked to a regulatory region of DNA that directed transcription in endosperm.
D) The wild-type genes were mutated and new mutant alleles that are expressed only in endosperm were selected.
Answer: C
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