Describe the steps involved in cytogenetic harvesting and banding, and explain the purpose of each
What will be an ideal response?
Answer:
Step 1: Arrest mitotically active cells in metaphase by incubating the sample with colchicine or colcemid that disrupts the spindle apparatus
Step 2: Incubate the mixture in a hypotonic solution (0.075M KCl) to lyse the erythrocytes and swell the nucleated cells
Step 3: Fix cells with Carnoy's fixative (3:1 mixture of methanol to glacial acetic acid)
Step 4: Make a trial slide by adding 3-4 drops of the final cell suspension mixture onto a clean glass slide
Step 5: Dry the trial slide and examine it by phase microscopy. If the first slide does not show optimal quality, the suspension can be concentrated or diluted to improve the slide's quality.
Step 6: "Aging" of the slide: Heat the slide in a 60 °C oven overnight to prepare for banding.
Step 7: Banding: stain chromosomes; Q-banding is done with quinacrine; G-banding is done with Giemsa stain
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In a patient with suspected bronchogenic carcinoma, how many biopsy specimens need to be collected during an endobronchial biopsy?
a. 1 b. 2 c. 3 d. 4
List four examples or types of outpatient encounters
What will be an ideal response?
This form of treatment causes cancer tumors to shrink.
A. Radiation therapy B. Chemotherapy C. Immunotherapy D. Acupressure
A respiratory therapist is weaning a patient from CPAP as she shows signs of clinical improvement. FIO2 should be decreased in 0.05 decrements until which of the following levels are reached?
A) 0.20 – 0.40 B) 0.40 – 0.60 C) 0.60 – 0.70 D) 0.60 – 0.80