Any tissues received in the clinical microbiology laboratory should be minced and homogenized in a biosafety cabinet. If lung tissue is sent and processed, then which organism should be ex-amined using direct fluorescent antibody (DFA) testing?
a. Listeria
b. Legionella
c. Leishmania
d. Leptospira
B
Tissue may be inoculated to tissue culture cells for isolation of viruses and should be manipulated in a laminar flow biologic safety cabinet. Brain, lung, spinal fluid, and blood are generally good specimens for viral isolation. Tissue may be examined by immunofluorescence for the presence of herpes simplex virus, varicella-zoster virus, cytomegalovirus, or rabies viral particles. Lung tissue should be examined by DFA test for Legionella spp.
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Fill in the blank(s) with the appropriate word(s).
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