The following are steps involved in producing recombinant DNA using human DNA and a plasmid. Which one of the following answers best reflects the proper order of these steps?
1. select bacteria that contain human DNA
2. isolate and purify DNA to be recombined
3. add DNA ligase to join human DNA with plasmid
4. cut human DNA and plasmid with restriction enzyme
5. introduce new plasmid to bacteria
A) 4, 1, 5, 3, 2
B) 2, 4, 3, 5, 1
C) 2, 5, 1, 3, 4
D) 4, 3, 2, 1, 5
E) 5, 1, 2, 4, 3
B
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