A newly discovered prokaryote produces a compound with promising antimicrobial effects. Devise a set of tests to determine whether the antimicrobial is broad or narrow spectrum and bactericidal or bacteriostatic
What will be an ideal response?
The following includes elements which should be present. The spectrum of action can be determined by testing the antimicrobial against representatives of the various groups of pathogenic bacteria: Gram positives such as Staphylococcus, Streptococcus, and Bacillus, members of the Gram-negative Enterobacteriaceae and other Gram-negative bacteria (Neisseria, for example), mycobacteria, and chlamydias. These tests might be done on broth cultures as MIC tests, or plate cultures as Etests. One or more broad spectrum antibiotics should be included as controls. Once susceptible microbes have been identified, MBC tests can be conducted to determine whether the antimicrobial is bacteriostatic or bactericidal. Appropriate controls should be included.
Bloom's Taxonomy: Synthesis
Section: Mechanisms of Antimicrobial Action
Learning Outcome: 10.14, 10.15
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