Proper storage of cerebrospinal fluid (CSF) sent to the laboratory for bacterial culture includes:
a. refrigeration for up to 23 hours.
b. freezing at 70°C.
c. incubation at 35°C.
d. freezing at temperatures above 70°C.
C
CSF should be immediately hand-delivered to the laboratory. Specimens should never be refri-gerated. Certain agents, such as Streptococcus pneumoniae, may not be detectable after an hour or longer unless antigen detection methods are used. If not rapidly processed, then CSF should be incubated (35°C) or left at room temperature. One exception to this rule involves CSF for viral studies. These specimens may be refrigerated for as long as 23 hours after collection or frozen at -70°C if a longer delay is anticipated until they are inoculated. CSF for viral studies should never be frozen at temperatures above -70°C.
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