Define the terms restriction enzyme and ligase, and describe how these enzymes are used in recombinant DNA
technology. What will be an ideal response?
Restriction enzymes are endonucleases with specificity for certain base sequences.
Restriction enzymes enable scientists to cut both vector DNA and DNA of interest (for
example, human DNA) to create compatible ends that can be used to form a recombinant
plasmid. DNA ligase catalyzes the formation of covalent bonds between two DNA
molecules, such as in DNA replication. This enzyme is used to link the vector DNA with the
DNA of interest.
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