AST was performed using Mueller-Hinton agar with 5% sheep blood, 35°C in 5% CO2 from 24 hours according to standard protocols, what is the most likely identity of the organism being tested?
a. S. aureus
b. Streptococcus pneumoniae
c. Neisseria gonorrhoeae
d. Moraxella catarrhalis
B
Standardized conditions for AST by disk diffusion of Streptococcus spp. require M-H media supplemented with 5% sheep blood and enriched atmosphere for 20 to 24 hours. S. aureus does not require specialized media (although 2% NaCl can be incorporate to aid in the detection of oxacillin-R). Routine susceptibility testing is not usually done on N. gonorrhoeae or M. cata-rrhalis. If AST is done on N. gonorhoeae, then growth supplements are added and incubated in 5% CO2.
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What will be an ideal response?