When using bacteria to clone a human gene and express its product, as in the case of insulin, the gene cannot contain introns because bacteria do not have the enzymes to process mRNA. How do scientists overcome this challenge?
What will be an ideal response?
Reverse transcriptase is used to "reversely transcribe" human mRNA to DNA. The DNA is referred to as cDNA and does not contain introns. Bacteria may then transcribe and translate the cloned cDNA to produce human protein because the genetic code is the same in humans and bacteria.
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