The methanol in Wright stain:
a. Establishes the alkaline pH need for staining
b. Fixes the cells
c. Establishes the acid pH needed for staining
d. Serves no function except as a solvent for the dyes
ANS: B
It is important that cell structures be stabilized before staining. If not, then the cells will be lysed when the buffer is added and thus will not adhere to the slide. Methanol in the stain fixes the cells to provide this stabilization of cell structure.
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