The Human Microbiome Project is a large research program that aims to understand all of the microorganisms on and in the human body. Which "-omic" methods could be applied directly to a sample from the human body to study the microorganisms in t

Propose a general experimental approach for analyzing a sample containing a complex mixture of microorganisms.
What will be an ideal response?


Answer: A sample from the human body, such as stool or tooth swab, would be collected and the DNA or RNA extracted. RNA would be converted to cDNA. The cDNA would then be sequenced. This approach is metagenomics (and metatransciptomics for RNA). After sequencing, the short sequences must be assembled into longer stretches (genes or whole genomes). An alternative answer could outline the procedure of single-cell genomics from cells obtained from samples from the human body or the use of a gene chip for identification of organisms. Any answer that shows understanding of general steps and uses terms appropriately and consistently throughout the answer would be acceptable.

Biology & Microbiology

You might also like to view...

Which of the following is/are true about monoclonal antibodies?

A. They recognize a specific epitope B. They are a useful tool in disease diagnosis C. They are made by hybridizing a myeloma cell and an activated B cell D. All of the choices are correct.

Biology & Microbiology

A(n) ________ forms during cytokinesis as contractile filaments in the cell tighten

Fill in the blank(s) with correct word

Biology & Microbiology

Plate tectonics explain that the amount of Earth being formed is much greater than the amount of the Earth's crust being destroyed in subduction zones.

Answer the following statement true (T) or false (F)

Biology & Microbiology

Typically, when one wishes to represent a gene, the symbol used is ________

A) in all upper case letters B) in bold print C) in italics D) in all lowercase letters E) underlined

Biology & Microbiology