You want to insert your favorite gene, which has been digested with Ncol endonuclease on both ends, into a vector. Ncol recognizes the DNA sequence C*CATGG. The vector you want to use contains many endonuclease recognition sites, but does not include an Ncol site. Which restriction endonuclease would be the most suitable replacement to digest the vector?
A. Kpnl - GGTAC*C
B. Sunl - C*GTACG
C. Sphl - G*CATGC
D. CViAll - C*ATG
Answer: C
You might also like to view...
In the Gulf of Mexico, red tides caused by the dinoflagellate Karenia brevis cause massive fish kills as well as respiratory problems in humans. As an environmental scientist, what would you suggest concerned citizens do to help prevent these red tides?
A. Purchase fuel-efficient vehicles B. Decrease the amount of artificial light in coastal areas C. Encourage sustainable fishing D. Reduce fertilizer use on lawns and gardens E. Participate in beach cleanup efforts
All of the following choices delay or block expression of a gene except ________
a. expression of a microRNA complementary in sequence to the gene b. the binding of a repressor to certain sites on DNA c. methylation of DNA d. the binding of an activator to a promoter
Explain why there is a difference in the mechanism by which an animal cell and a plant cell accomplishes cytokinesis
What will be an ideal response?
After their absorption from the gut lumen, monoglycerides and fatty acids are rejoined in the villi epithilia cells, forming lipid droplets called
What will be an ideal response?