In the Sanger, or chain termination, method of DNA sequencing, incorporation of the nucleotides stops because

A) the dideoxynucleotide analogs have no 3?-OH group to continue DNA synthesis.
B) the dideoxynucleotide analogs have no 5?-OH group to continue DNA synthesis.
C) the dideoxynucleotide analogs have no 2?-OH group to continue DNA synthesis.
D) the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
E) the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.


Answer: A

Biology & Microbiology

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