The restriction enzyme SmaI cuts DNA at the 6-base recognition sequence 5'-CCCGGG-3'. Calculate the frequency of occurrence of this sequence. (Assume a 50% GC content.)

What will be an ideal response?


Assuming a 1:1 AT:CG ratio in the genome and a random distribution of nucleotides, each nucleotide pair will occur with equal frequency ( ). The 6-nucleotide sequence would then occur with a frequency of ( )6, 0.00024, cutting once in every 4,096 base pairs.

Biology & Microbiology

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While innate immunity is ancient and universal amongst animals, the adaptive immune system is generally considered to be unique to the vertebrates. However, some researchers have suggested, with much controversy, that invertebrates may have a form of adaptive immunity as well. What sort of evidence would be required to fully demonstrate such a claim? (Check all that apply.)  

_____  B and T cells _____  CD4 receptors _____  efficacy of vaccination _____  highly variable molecules that can bind various antigens _____  identified cells that are necessary to provide acquired immunity _____  immunoglobulin molecules _____  resistance upon second exposure to a pathogen _____  specific resistance to one strain but not another after exposure _____  thymus gland _____  Toll-like receptors

Biology & Microbiology

How are DNA and RNA similar?

a. They are both comprised of long chains of nucleotides. b. They are both comprised of deoxyribonucleic acid. c. They are both comprised of long chains of glucose. d. None of the above.

Biology & Microbiology

Charles Darwin advocated a theory of evolution that involved individuals acquiring useful characteristics during their lifetimes

Indicate whether the statement is true or false

Biology & Microbiology

Which foods or food ingredients are regulated by the FDA to ensure food safety?

A) vitamin tablets B) cheese C) sirloin steaks D) chicken wings

Biology & Microbiology