A Giemsa stain is performed on an eye exudate on a patient with calabar swelling, eosinophilia, and a recent travel history to Africa. L. loa is suspected; however, the sheath in the adult worm recovered does not stain. Which one of the following statements explains this discrepancy?

a. The worm recovered is not L. loa.
b. The specimen should have been stained with trichrome stain.
c. The Giemsa stain is most likely expired.
d. No discrepancy is present. The sheath does not stain with Giemsa stain.


D
The identification of the adult worm from the eye, in tissue, or in the peripheral blood provides a definitive diagnosis. The L. loa organism contains a sheath that does not stain with Giemsa. The adult females are larger than the adult males. The nuclei extend to the tail in an irregularly ar-ranged fashion.

Biology & Microbiology

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