In the Sanger method of DNA sequencing, incorporation of the nucleotides stops because
A) the dideoxynucleotide analogs have no 3'-OH group to continue DNA synthesis.
B) the dideoxynucleotide analogs have no 5'-OH group to continue DNA synthesis.
C) the dideoxynucleotide analogs have no 2'-OH group to continue DNA synthesis.
D) the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
E) the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.
A) the dideoxynucleotide analogs have no 3'-OH group to continue DNA synthesis.
You might also like to view...
In humans, the front of the larynx is commonly referred to as
(a) the voice box. (b) the Adam’s apple. (c) the food pipe. (d) both a and b
Natural selection is based on all of the following except:
A. the best-adapted individuals tend to leave the most offspring B. genetic variation exists within populations C. populations tend to produce more individuals than the environment can support D. individuals adapt to their environments and thereby, evolve E. individuals who survive longer tend to leave more offspring than those who die young
The first Drosophila segmentation genes to act are the __________, which begin organization of the
body into regions. a. pair-rule genes b. segment polarity genes c. gap genes d. homeotic genes e. maternal effect gene
A researcher follows the mutation rates in the nematode Caenorhabditis elegans in five different tissue types across 300 generations. One would expect mutation rates to be ________ across the different tissue types
a. equal b. approximately equal c. different d. deleterious e. None of the above