generate cDNA libraries for the same cell line under different conditions. When I compare the
libraries, I find some cDNAs in one library that are missing from the other. What is the best
explanation for this result?
a. One of the cDNA libraries is really a genomic library.
b. Even the same cell type will change gene expression as conditions change. The difference
in cDNAs tells me which genes are expressed in each set of conditions.
c. There was an error in generating one of the cDNA libraries and some of the sequences
were not cloned properly.
d. The probe I used in the Southern blot of the libraries must have been poorly constructed.
e. There is no explanation other than experimental error.
B
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You are preparing to administer the first of two units of packed RBCs. Evaluate each of the following statements about the safe administration of blood.
Answer T or F. Discuss why the false statements are incorrect. 1. Prime the correct tubing and filter with normal saline. 2. Verify K.L.'s identification with the secretary in the endoscopy suite. 3. Obtain baseline vital signs before starting the transfusion. 4. Begin the transfusion at a rate of 125ml per hour 5. Take K.L.'s vital signs 30 minutes after starting the transfusion. 6. Complete the transfusion within 6 hours of receiving the unit.
Members of this Lophotrochozoan phylum include earthworms, bristle worms, and leeches
a. Rotifera b. Mollusca c. Platyhelminthes d. Annelida e. Nemetera
Polyploidy may arise from the failure of chromosomes to separate during cell division or by the
fertilization of an egg by more than one sperm. _________________ Indicate whether the statement is true or false.
In which example are the proximate cause and the ultimate cause of morphological evolution correctly matched?
A. Proximate: point mutations in protein coding genes affect protein function and structure, ultimate: chemical and physical configuration of a protein affect its function B. Proximate: cis-regulatory elements are found upstream of a gene; ultimate: cis regulation is required to activate a gene C. Proximate: natural selection causes morphology to differ among species, ultimate: natural selection prevents morphological variation within a species D. Proximate: changes in gene regulation lead to changes in morphology; ultimate: natural selection favors morphologies that work best in their environment E. Proximate: natural selection favors gene combinations that have high fitness in their environment; ultimate: genetic changes alter morphology and fitness