Cells must be lysed to release the protein of interest if ________
A) the protein is secreted into the media
B) the protein is intracellular
C) the protein is hydrophobic
D) the protein is positively charged
E) the protein is hydrophilic
B
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Sporadic cancers ____
a. may develop when proto-oncogenes mutate to form tumor suppressor genes b. may develop when tumor suppressor genes are over-expressed c. are caused by benign tumors d. are cancers that are not inherited e. are less frequent than familial cancers
Why do normal nucleotides need to be included in the Sanger sequencing reaction mix?
a. so the DNA polymerase has a substrate to work with b. to enhance the action of the dideoxynucleotides c. so a longer sequence (up to 300 additional base pairs) can be determined than without (normally 200 bp). d. so multiple sequences of different lengths will be generated e. to prevent the DNA polymerase from making too many errors
Opportunistic pathogens
A. cause disease in every individual. B. are always pathogens. C. cause disease in compromised individuals. D. have well-developed virulence factors.
An adult stem cell differs from an embryonic stem cell in that it is not
A. totipotent. B. capable of further cell divisions. C. able to accept methyl groups. D. able to produce a blastocyst.