Why do normal nucleotides need to be included in the Sanger sequencing reaction mix?
a. so the DNA polymerase has a substrate to work with
b. to enhance the action of the dideoxynucleotides
c. so a longer sequence (up to 300 additional base pairs) can be determined than without
(normally 200 bp).
d. so multiple sequences of different lengths will be generated
e. to prevent the DNA polymerase from making too many errors
D
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