Define the purpose of inserting a gene into a plasmid cloning vector
What will be an ideal response?
ANSWER: After inserting a gene into a cloning vector, the recombinant plasmid can be transformed into
bacterial host cells. Here, hundreds of copies of the gene can be generated very easily by DNA replication when the bacteria divide. This allows the gene or DNA of interest to be
produced in amounts that can then be used in other applications, such as restriction enzyme
digestions or sequencing. If the protein-coding region of a gene is combined with an
expression vector, mass production of its protein product is possible by transcription and
translation in the host cell.
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