Double-stranded DNA absorbs less UV light than single-stranded DNAbecause:

A. base stacking in double-stranded DNA increases the absorbance of white light and causes a decrease in the amount of UV light it can absorb. This decrease in UV absorbance is called the hyperchromic effect.
B. the bases are on the inside of the helix, inaccessible to the UV light in double-stranded
DNA.
C. in single-stranded DNA the bases are not interacting so more photons can be absorbed by the pentose rings.
D. single-stranded DNA actually absorbs more of the UV light, making less light available to double-stranded DNA.
E. of the hypochromic effect that results when hydrogen bonding and base stacking in double-stranded DNA limits resonance in the aromatic rings of the bases.


E

Biology & Microbiology

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